daily work in molecular biology is currently dependent on a large number of computing devices. Deep, large-scale studies of the ‘ecosystem’ Web tool, characteristics, interconnectivity, usage patterns / quotes, temporal evolution and the level of damage is very important to understand the forces that shape and to inform initiatives aimed at funding, long-term maintenance and repair. In particular, long-term maintenance of the tool is interrupted because of their specific development model. Hundreds of published studies be irreproducible de facto, as the software used to make them become unavailable. In this study, we present a large-scale survey> 5400 publication describes the Web server in two major bibliographic sources to disseminate new software developments in molecular biology.

For all these servers, we studied their pattern citation, the subjects they tackle, their citation network and temporal evolution of these factors. We also analyze how these factors affect the availability of this server (whether they are still alive). Our results indicate that these ecosystems are highly interconnected tools and adapt to the ‘trendy’ subjects in every moment. The server presents a characteristic temporal patterns quote / use, and there is an alarming level server ‘death’, which is influenced by factors such as the popularity of server and institutions that host it. These results can inform initiatives aimed at long-term maintenance of these resources.

Filoviridae are a family of negative-strand RNA viruses that includes several important human pathogens. Ebola virus (EBOV) and Marburg viruses are well known filoviruses that cause life-threatening viral hemorrhagic fever in humans and nonhuman primates. In addition to the pathogenesis of severe, filoviruses also showed a tendency to spread from human to human through close contact, posing a challenge for the containment and crisis management.

outbreaks of the past, especially recently EBOV epidemic of West Africa, has been responsible for thousands of deaths and filoviruses leapfrog into the public consciousness. Both national and international health agencies continue to consider potential outbreaks Filovirus as a major threat to global public health. To develop effective countermeasures, a basic understanding of the biology Filovirus required. This review covers the epidemiology, ecology, molecular biology, and evolution filoviruses.

molecular evolution between chemistry and biology: Interaction of competition, cooperation, and the mutation.

Biological evolution is reduced to three fundamental processes in the spirit of the model at a minimum: (i) competition caused by differential fitness, (ii) cooperation competitor in the sense of symbiosis, and (iii) the variation introduced by mutation understood as reproduction error prone.

Three combinations of two basic processes respectively, ([Formula: see text]) competition and mutation, ([Formula: see text]) cooperation and competition, and ([Formula: see text]) cooperation and mutations, were analyzed. Changes in population dynamics caused by bifurcations and threshold phenomena are discussed.

Hydrogen Peroxide Assay Kit
NAT1002	National Diagnostics	1KIT	EUR 308.4

ProtoBlock System
NAT1004	National Diagnostics	1KIT	EUR 122.4

450ML PBS 10X
NAT1006	National Diagnostics	450ML	EUR 96

4L PBS 10X
NAT1008	National Diagnostics	4L	EUR 238.8

ProtoGlow ECL 200ml Kit
NAT1010	National Diagnostics	EACH	EUR 354

ProtoGlow ECL 500ml Kit
NAT1012	National Diagnostics	EACH	EUR 627.6

100G Acrylamide
NAT1014	National Diagnostics	100G	EUR 120

500G Acrylamide
NAT1016	National Diagnostics	500G	EUR 261.6

1KG Acrylamide
NAT1018	National Diagnostics	1KG	EUR 412.8

25G AquaPor LE
NAT1020	National Diagnostics	25G	EUR 132

100G AquaPor LE GTAC
NAT1022	National Diagnostics	100G	EUR 250.8

500G AquaPor LE GTAC
NAT1024	National Diagnostics	500G	EUR 878.4

25G AquaPor ES
NAT1026	National Diagnostics	25G	EUR 152.4

100G AquaPor ES
NAT1028	National Diagnostics	100G	EUR 350.4

25G AquaPor LM
NAT1030	National Diagnostics	25G	EUR 288

100G AquaPor LM
NAT1032	National Diagnostics	100G	EUR 860.4

25G AquaPor HR
NAT1034	National Diagnostics	25G	EUR 236.4

100G AquaPor HR
NAT1036	National Diagnostics	100G	EUR 592.8

25G AquaPor 3:1
NAT1038	National Diagnostics	25G	EUR 250.8

100G AquaPor 3:1
NAT1040	National Diagnostics	100G	EUR 570

25G Bis (Methylene Bis-Acrylamide)
NAT1042	National Diagnostics	25G	EUR 100.8

25G DATD Ultrapure
NAT1044	National Diagnostics	25G	EUR 168

250G Glycine
NAT1046	National Diagnostics	250G	EUR 94.8

1KG Glycine Ultrapure
NAT1048	National Diagnostics	1KG	EUR 128.4

250G Tris
NAT1050	National Diagnostics	250G	EUR 102

1KG Tris Ultrapure
NAT1052	National Diagnostics	1KG	EUR 177.6

100G Tricine
NAT1054	National Diagnostics	100G	EUR 120

100G Ion Exchange Resin (mixed bed)
NAT1056	National Diagnostics	100G	EUR 96

25G Riboflavin
NAT1058	National Diagnostics	25G	EUR 96

25ML TEMED
NAT1060	National Diagnostics	25ML	EUR 99.6

25G Ammonium Persulfate
NAT1062	National Diagnostics	25G	EUR 96

100G Ammonium Persulfate
NAT1064	National Diagnostics	100G	EUR 118.8

1G Dithiothreitol (DTT)
NAT1066	National Diagnostics	1G	EUR 103.2

5G Dithiothreitol (DTT)
NAT1068	National Diagnostics	5G	EUR 183.6

50ML 2-Mercaptoethanol
NAT1070	National Diagnostics	50ML	EUR 112.8

100G SDS Ultrapure
NAT1072	National Diagnostics	100G	EUR 111.6

1KG SDS Ultrapure
NAT1074	National Diagnostics	1KG	EUR 457.2

250G Urea
NAT1076	National Diagnostics	250G	EUR 98.4

1KG Urea
NAT1078	National Diagnostics	1KG	EUR 138

450ML Glycerol Ultrapure
NAT1080	National Diagnostics	450ML	EUR 132

200ML Tween 20 Ultrapure
NAT1082	National Diagnostics	200ML	EUR 132

1L Tween 20
NAT1084	National Diagnostics	1L	EUR 169.2

200ML Formamide
NAT1086	National Diagnostics	200ML	EUR 111.6

500ML Boric Acid
NAT1088	National Diagnostics	500ML	EUR 103.2

100G EDTA
NAT1090	National Diagnostics	100G	EUR 94.8

500G EDTA
NAT1092	National Diagnostics	500G	EUR 177.6

GelDry Film 11 X 12 cm
NAT1094	National Diagnostics	PK50	EUR 100.8

25ML Formamide CE
NAT1096	National Diagnostics	25ML	Ask for price

25ML Glass Bond
NAT1098	National Diagnostics	25ML	EUR 158.4

450ML Glass Free
NAT1100	National Diagnostics	450ML	EUR 196.8

GelDry Film 22.5 X 22.5 cm
NAT1102	National Diagnostics	PK50	EUR 130.8

1L DEPC Treated Water
NAT1104	National Diagnostics	1L	EUR 120

Sterling Rapid Silver Stain Kit
NAT1106	National Diagnostics	1KIT	EUR 265.2

1L ProtoBlue Safe
NAT1108	National Diagnostics	1L	EUR 159.6

4L ProtoBlue Safe
NAT1110	National Diagnostics	4L	EUR 282

1L ProtoStain Blue
NAT1112	National Diagnostics	1L	EUR 164.4

25ML Nuclistain
NAT1114	National Diagnostics	25ML	EUR 128.4

450ML AcrylaGel 30%
NAT1116	National Diagnostics	450ML	EUR 128.4

1L AcrylaGel 30%
NAT1118	National Diagnostics	1L	EUR 177.6

450ML Bis-AcrylaGel 2%
NAT1120	National Diagnostics	450ML	EUR 98.4

1L Bis-AcrylaGel 2%
NAT1122	National Diagnostics	1L	EUR 111.6

450ML UreaGel 29:1 Concentrate
NAT1124	National Diagnostics	450ML	EUR 133.2

1L UreaGel 29:1 Concentrate
NAT1126	National Diagnostics	1L	EUR 183.6

UreaGel 29:1 System 1L Kit
NAT1128	National Diagnostics	EACH	EUR 171.6

UreaGel 29:1 System 2.2L Kit
NAT1130	National Diagnostics	EACH	EUR 253.2

450ML SequaGel UreaGel Concentrate
NAT1132	National Diagnostics	450ML	EUR 126

1L SequaGel UreaGel Concentrate
NAT1134	National Diagnostics	1L	EUR 160.8

SequaGel Sequencing System 1L Kit
NAT1136	National Diagnostics	1KIT	EUR 171.6

SequaGel Sequencing System 2.2L Kit
NAT1138	National Diagnostics	EACH	EUR 253.2

100ML SequaGel Buffer
NAT1140	National Diagnostics	100ML	EUR 80.4

200ML SequaGel UreaGel Buffer
NAT1142	National Diagnostics	200ML	EUR 84

450ML SequaGel UreaGel-6
NAT1144	National Diagnostics	450ML	EUR 115.2

1L SequaGel UreaGel-6
NAT1146	National Diagnostics	1L	EUR 151.2

450ML SequaGel UreaGel-8
NAT1148	National Diagnostics	450ML	EUR 115.2

1L SequaGel UreaGel-8
NAT1150	National Diagnostics	1L	EUR 151.2

450ML SequaGel UreaGel Diluent
NAT1152	National Diagnostics	450ML	EUR 90

1L SequaGel UreaGel Diluent
NAT1154	National Diagnostics	1L	EUR 103.2

90ML SequaGel Complete Buffer
NAT1156	National Diagnostics	90ML	EUR 79.2

200ML SequaGel UreaGel Complete Buffer
NAT1158	National Diagnostics	200ML	EUR 85.2

450ML SequaGel XR
NAT1160	National Diagnostics	450ML	EUR 126

1L SequaGel XR
NAT1162	National Diagnostics	1L	EUR 165.6

100ML SequaGel XR Concentrate
NAT1164	National Diagnostics	100ML	EUR 128.4

450ML SequaGel XR Concentrate
NAT1166	National Diagnostics	450ML	EUR 361.2

200ML SequaGel MD Monomer Solution
NAT1168	National Diagnostics	200ML	EUR 220.8

SequaGel MD SSCP Kit
NAT1170	National Diagnostics	1KIT	EUR 253.2

SequaGel MD Heteroduplex Kit
NAT1172	National Diagnostics	EACH	EUR 265.2

1.2ML SequaGel MD SSCP Stop Solution
NAT1174	National Diagnostics	1.2ML	EUR 85.2

450ML AccuGel 19:1 (30%)
NAT1176	National Diagnostics	450ML	EUR 126

1L AccuGel 19:1 (30%)
NAT1178	National Diagnostics	1L	EUR 165.6

450ML AccuGel 19:1 (40%)
NAT1180	National Diagnostics	450ML	EUR 130.8

1L AccuGel 19:1 (40%)
NAT1182	National Diagnostics	1L	EUR 168

450ML AccuGel 29:1 (30%)
NAT1184	National Diagnostics	450ML	EUR 126

1L AccuGel 29:1 (30%)
NAT1186	National Diagnostics	1L	EUR 165.6

450ML AccuGel 29:1 (40%)
NAT1188	National Diagnostics	450ML	EUR 130.8

1L AccuGel 29:1 (40%)
NAT1190	National Diagnostics	1L	EUR 168

1.2ML Triple Dye Loading Buffer (6X)
NAT1192	National Diagnostics	1.2ML	EUR 85.2

UreaGel Loading Buffer
NAT1194	National Diagnostics	10X1ML	EUR 222

1L TBE 10X
NAT1196	National Diagnostics	1L	EUR 98.4

4L TBE 10X
NAT1198	National Diagnostics	4L	EUR 145.2

1L TBE 5X
NAT1200	National Diagnostics	1L	EUR 92.4

4L TBE 5X
NAT1202	National Diagnostics	4L	EUR 111.6

25ML TE BUFFER 100X
NAT1204	National Diagnostics	25ML	EUR 98.4

25ML 10X CE Buffer
NAT1206	National Diagnostics	25ML	EUR 138

100ML 10X CE Buffer
NAT1208	National Diagnostics	100ML	EUR 196.8

450ML 20X Tris-MOPS SDS
NAT1210	National Diagnostics	450ML	EUR 130.8

450ML 20X Tris-MEPS SDS
NAT1212	National Diagnostics	450ML	EUR 130.8

1L Tris-Tricine-SDS PAGE Buffer (10X)
NAT1214	National Diagnostics	1L	EUR 217.2

1L Tris-Glycine-SDS PAGE Buffer (10X)
NAT1216	National Diagnostics	1L	EUR 105.6

4L Tris-Glycine-SDS PAGE Buffer (10X)
NAT1218	National Diagnostics	4L	EUR 148.8

1L TTE Glycerol Tolerant Buffer 20X
NAT1220	National Diagnostics	1L	EUR 160.8

1L TAE Buffer 50X
NAT1222	National Diagnostics	1L	EUR 148.8

1L SSC Buffer 20X
NAT1224	National Diagnostics	1L	EUR 98.4

4L SSC Buffer 20X
NAT1226	National Diagnostics	4L	EUR 174

100ML SDS Solution 20%
NAT1228	National Diagnostics	100ML	EUR 92.4

450ML SDS Solution 20%
NAT1230	National Diagnostics	450ML	EUR 138

1L Denaturation Solution
NAT1232	National Diagnostics	1L	EUR 102

1L Neutralization Solution
NAT1234	National Diagnostics	1L	EUR 102

50G Dextran Sulfate
NAT1236	National Diagnostics	50G	EUR 193.2

250G Dextran Sulfate
NAT1238	National Diagnostics	250G	EUR 524.4

1L Tris-Glycine Electroblotting Buffer 10X
NAT1240	National Diagnostics	1L	EUR 102

4L Tris-Glycine Electroblotting Buffer 10X
NAT1242	National Diagnostics	4L	EUR 145.2

1L TBS (10X) Tris Buffered Saline
NAT1244	National Diagnostics	1L	EUR 126

1L TBST (10X) Tris Buffered Saline w/Tween 20
NAT1246	National Diagnostics	1L	EUR 126

ProtoGel Sample Prep Kit
NAT1248	National Diagnostics	EACH	EUR 223.2

Protein Loading Buffer Blue 2X
NAT1250	National Diagnostics	10X1ML	EUR 105.6

Protein Loading Buffer 5X
NAT1252	National Diagnostics	10X1ML	EUR 151.2

50ML Protein Precipitation Kit
NAT1254	National Diagnostics	50ML	EUR 270

100ML ProtoLift Western Strip Buffer
NAT1256	National Diagnostics	100ML	EUR 121.2

450ML ProtoGel (30%)
NAT1258	National Diagnostics	450ML	EUR 136.8

1L ProtoGel (30%)
NAT1260	National Diagnostics	1L	EUR 187.2

450ML ProtoGel (40%)
NAT1262	National Diagnostics	450ML	EUR 157.2

1L ProtoGel (40%)
NAT1264	National Diagnostics	1L	EUR 204

450ML ProtoGel Resolving Buffer 4X
NAT1266	National Diagnostics	450ML	EUR 100.8

1L ProtoGel Resolving Buffer
NAT1268	National Diagnostics	1L	EUR 128.4

200ML ProtoGel Stacking Buffer
NAT1270	National Diagnostics	200ML	EUR 92.4

100ML ProtoGel Quick-Cast 12%
NAT1272	National Diagnostics	100ML	EUR 105.6

450ML ProtoGel Quick-Cast 12%
NAT1274	National Diagnostics	450ML	EUR 223.2

0.5ML Insite Markers
NAT1276	National Diagnostics	0.5ML	EUR 238.8

0.5ML ProtoMarkers
NAT1278	National Diagnostics	0.5ML	EUR 236.4

ProtoMetrics
NAT1280	National Diagnostics	EACH	EUR 242.4

1L EDTA 0.5M Solution
NAT1282	National Diagnostics	1L	EUR 183.6

1L Sodium Chloride 0.009 Solution
NAT1284	National Diagnostics	1L	EUR 140.4

1L Sodium Chloride 1M Solution
NAT1286	National Diagnostics	1L	EUR 140.4

1L Potassium Chloride 1M Solution
NAT1288	National Diagnostics	1L	EUR 140.4

1L Tris-HCL 1M pH 7.2
NAT1290	National Diagnostics	1L	EUR 126

1L Tris-HCL 1M pH 7.4
NAT1292	National Diagnostics	1L	EUR 126

1L Tris-HCL 1M pH 7.6
NAT1294	National Diagnostics	1L	EUR 126

Histosol
NAT1296	National Diagnostics	EACH	EUR 106.8

Histosol
NAT1298	National Diagnostics	EACH	EUR 270

Mirskys Fixative (ready-to-use)
NAT1300	National Diagnostics	1USGALLON	EUR 96

Mirskys Fixative (ready-to-use)
NAT1302	National Diagnostics	EACH	EUR 228

200ML Mirskys Fixative
NAT1304	National Diagnostics	200ML	EUR 92.4

2L Mirskys Fixative
NAT1306	National Diagnostics	2L	EUR 134.4

100ML Histomount
NAT1308	National Diagnostics	100ML	EUR 86.4

450ML Histomount
NAT1310	National Diagnostics	450ML	EUR 111.6

Calci-Clear
NAT1312	National Diagnostics	EACH	EUR 93.6

Calci-Clear
NAT1314	National Diagnostics	EACH	EUR 153.6

Calci-Clear
NAT1316	National Diagnostics	EACH	EUR 463.2

Calci-Clear Rapid
NAT1318	National Diagnostics	EACH	EUR 90

Calci-Clear Rapid
NAT1320	National Diagnostics	EACH	EUR 153.6

Calci-Clear Rapid
NAT1322	National Diagnostics	EACH	EUR 463.2

DAMBE is a comprehensive software package for the analysis of genomic data and phylogenetic on Windows, Linux, and Macintosh. new functionality including imputing distance and phylogeny lost simultaneously (paving the way for building a phage great and trees transposons), bootstrap new / jackknifing method for PhyPA (phylogenetic of partiality in pairs), and improved functionality for fast and accurate estimation of the parameters of the form of the gamma distribution for fitting degree of heterogeneity on the site.