Molecular biology and structure of a novel penaeid shrimp densovirus elucidate convergent parvoviral host capsid evolution

Black tiger shrimp (Penaeus monodon) is a comprehensive decapod crustaceans raised for human consumption. Currently, the virus from two different lineages of parvovirus (PV, family Parvoviridae; subfamily Hamaparvovirinae) infects penaeid shrimp.

Here, the PV was isolated and cloned from Vietnam specimens of P. monodon, Penaeus monodon appointed metallodensovirus (PmMDV). This was the first member of a different lineage third proved to infect penaeid decapods. PmMDV has the unique transcription strategy among PV invertebrates, extensive use of alternative splicing and transcription incorporating elements characteristic of PV-infect vertebrates. The PmMDV protein has no significant sequence similarity with other PV, except for the helicase domain SF3 its nonstructural protein.

The capsid structure, determined by cryoelectron microscope for 3-Å resolution, has a surface morphology similar to Penaeus stylirostris densovirus, despite the lack of significant capsid viral proteins (VP) of sequence similarity. Unlike other PV, VP crease PmMDV without combining βA multimer strand and shown a unique interaction, including the incorporation of cations Ca2 +, attach the N termini below fivefold icosahedral symmetry axis and forming a basket-like pentamer helix bundle. While the order PmMDV VP does not have a canonical domain of phospholipase A2, the structure of a capsid EDTA-treated, determined to 2.8-Å resolution, suggesting a mechanism cation-dependent membrane-penetrating alternative in the N-terminal region.

PmMDV is observed examples of convergent evolution between invertebrates PV capsid structure in connection with the host-driven and unique as PV shows the structure of the cation-sensitive cart / egress endosomal depending on the alternative. Bioinformatics is a very resourceful tool for understanding the evolution of membrane proteins, such as transient receptor potential channel. bioinformatics expert users rely on special scripting and programming skills. Some web servers and standalone tools are available to users nonadvanced is willing to develop projects to understand their system of choice.

 Molecular biology and structure of a novel penaeid shrimp densovirus elucidate convergent parvoviral host capsid evolution
Molecular biology and structure of a novel penaeid shrimp densovirus elucidate convergent parvoviral host capsid evolution

molecular biology and evolution of cancer: from discovery to action.

Cancer development is an evolutionary process. During this process, the growing population of cancer cells undergo limit the ecological niches in the body, such as primary tumor, circulatory system, and metastatic sites vary. heterogeneous population of cancer cells undergo selection for adaptive phenotypes, which form the molecular genetic variations in the same genetic drift. cell lineage underwent convergent evolution towards a phenotype known as hallmarks of cancer that promote cancer initiation, growth, and metastasis.

Efforts to prevent or delay cancer evolution-and-development requires a deep understanding of the underlying process of molecular evolution. Here we discuss the suite concept and tools of the theory of evolution and ecology that can inform-and possibly change-cancer biology in new ways and means. These concepts and tools including comparative research on cancer throughout the different species and the application of phylogenetic approaches to analyze the evolution of tumor progression and metastasis.

Tetrahydrofuran Mix (THF) - 3 pack of 1.5mL ampules

ECS-B-047 1.5ML
EUR 92.34

BCIP (Molecular Biology Grade)

CE108 250 mg
EUR 75.6

BCIP (Molecular Biology Grade)

CE109 1 g
EUR 108

CHAPS (Molecular Biology Grade)

CE114 1 g
EUR 66

CHAPS (Molecular Biology Grade)

CE115 5 g
EUR 157.2

CHAPS (Molecular Biology Grade)

CE116 25 g
EUR 492

DAPI (Molecular Biology Grade)

CE117 5 mg
EUR 72

DAPI (Molecular Biology Grade)

CE118 25 mg
EUR 159.6

DAPI (Molecular Biology Grade)

CE119 100 mg
EUR 382.8

Dimethylsulfoxide (Molecular Biology Grade)

CE120 100 ml
EUR 66

Dimethylsulfoxide (Molecular Biology Grade)

CE121 500 ml
EUR 110.4

DTT (Molecular Biology Grade)

CE131 5 g
EUR 93.6

DTT (Molecular Biology Grade)

CE132 10 g
EUR 133.2

DTT (Molecular Biology Grade)

CE133 25 g
EUR 243.6

Glycine (Molecular Biology Grade)

CE158 1 kg
EUR 84

Glycine (Molecular Biology Grade)

CE159 5 kg
EUR 228

HEPES (Molecular Biology Grade)

CE171 100 g
EUR 98.4

HEPES (Molecular Biology Grade)

CE172 500 g
EUR 268.8

HEPES (Molecular Biology Grade)

CE173 1 kg
EUR 424.8

Lysozyme (Molecular Biology Grade)

CE188 1 g
EUR 70.8

Lysozyme (Molecular Biology Grade)

CE189 10 g
EUR 247.2

NAD (Molecular Biology Grade)

CE196 1 g
EUR 72

NAD (Molecular Biology Grade)

CE197 5 g
EUR 165.6

NBT (Molecular Biology Grade)

CE209 1 g
EUR 123.6

NBT (Molecular Biology Grade)

CE210 5 g
EUR 360

Tris (Molecular Biology Grade)

CE237 500 g
EUR 106.8

Tris (Molecular Biology Grade)

CE238 1 kg
EUR 153.6

Tris (Molecular Biology Grade)

CE239 5 kg
EUR 535.2

Tween20 (Molecular Biology Grade)

CE242 1 l
EUR 106.8

Water (Molecular Biology Grade)

CE243 500 ml
EUR 62.4

Water (Molecular Biology Grade)

CE244 1 l
EUR 67.2

100mL Molecular Biology Grade

46-000-CI PK6
EUR 70.68

500mL Molecular Biology Grade

46-000-CV PK6
EUR 124.26

Water, Ultrapure Molecular Biology Grade

41024-4L 4L
EUR 145.2
Description: Minimum order quantity: 1 unit of 4L

Ammonium sulfate (Molecular Biology Grade)

CE105 250 g
EUR 55.2

Ammonium sulfate (Molecular Biology Grade)

CE106 1 kg
EUR 72

Ammonium sulfate (Molecular Biology Grade)

CE107 5 kg
EUR 153.6

Bis-Acrylamid (Molecular Biology Grade)

CE110 50 g
EUR 94.8

Bis-Acrylamid (Molecular Biology Grade)

CE111 250 g
EUR 259.2

Formamide deionized (Molecular Biology Grade)

CE145 500 ml
EUR 87.6

Formamide deionized (Molecular Biology Grade)

CE146 1 l
EUR 120

Glycerol 87 % (Molecular Biology Grade)

CE154 1 l
EUR 93.6

Glycerol waterfree (Molecular Biology Grade)

CE155 500 ml
EUR 78

Glycerol waterfree (Molecular Biology Grade)

CE156 1 l
EUR 102

Glycerol waterfree (Molecular Biology Grade)

CE157 2.5 l
EUR 170.4

Guanidine - Hydrochloride (Molecular Biology Grade)

CE160 100 g
EUR 93.6

Guanidine - Hydrochloride (Molecular Biology Grade)

CE161 250 g
EUR 153.6

Guanidine - Hydrochloride (Molecular Biology Grade)

CE162 500 g
EUR 232.8

Guanidine - Hydrochloride (Molecular Biology Grade)

CE163 1 kg
EUR 352.8

Guanidine Thiocyanate (Molecular Biology Grade)

CE164 100 g
EUR 86.4

Guanidine Thiocyanate (Molecular Biology Grade)

CE165 500 g
EUR 192

Guanidine Thiocyanate (Molecular Biology Grade)

CE166 1 kg
EUR 307.2

Urea Crystalline (Molecular Biology Grade)

CE167 1 kg
EUR 72

Urea Crystalline (Molecular Biology Grade)

CE168 5 kg
EUR 181.2

MOPS buffer (Molecular Biology Grade)

CE194 100 g
EUR 102

MOPS buffer (Molecular Biology Grade)

CE195 250 g
EUR 169.2

Sodium chloride (Molecular Biology Grade)

CE205 500 g
EUR 62.4

Sodium chloride (Molecular Biology Grade)

CE206 1 kg
EUR 70.8

Sodium chloride (Molecular Biology Grade)

CE207 5 kg
EUR 123.6

D(+)-Sucrose (Molecular Biology Grade)

CE224 500 g
EUR 67.2

D(+)-Sucrose (Molecular Biology Grade)

CE225 1 kg
EUR 84

D(+)-Sucrose (Molecular Biology Grade)

CE226 5 kg
EUR 207.6

Tris - Hydrochloride (Molecular Biology Grade)

CE234 250 g
EUR 99.6

Tris - Hydrochloride (Molecular Biology Grade)

CE235 500 g
EUR 144

Tris - Hydrochloride (Molecular Biology Grade)

CE236 1 kg
EUR 223.2

TritonX-100 (Molecular Biology Grade)

CE240 500 ml
EUR 67.2

TritonX-100 (Molecular Biology Grade)

CE241 1 l
EUR 79.2

Tween 20, Molecular Biology Grade

T9100-010 100ml
EUR 86.4

Tween 20, Molecular Biology Grade

T9100-050 500ml
EUR 133.2

Tween 20, Molecular Biology Grade

T9100-100 1L
EUR 160.8

1L Molecular Biology Grade Water

46-000-CM PK6
EUR 177.84

Water, distilled, GlenBiol™, suitable for molecular biology

GK8512-1L 1 l
EUR 92.4

Agarose, low EEO, GlenBiol, suitable for molecular biology

GE6258-100G 100 g
EUR 217.2

Phenol, (Carbolic acid) Double distilled for Molecular Biology

PD0252 500g
EUR 192.59

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE135 250 g
EUR 72

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE136 500 g
EUR 86.4

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE137 1 kg
EUR 124.8

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE138 5 kg
EUR 418.8

D(+)-Glucose waterfree (Molecular Biology Grade)

CE148 500 g
EUR 67.2

D(+)-Glucose waterfree (Molecular Biology Grade)

CE149 1 kg
EUR 75.6

D(+)-Glucose waterfree (Molecular Biology Grade)

CE150 5 kg
EUR 180

Yeast extract powder (Molecular Biology Grade)

CE169 500 g
EUR 133.2

Hyaluronidase Grade I (Molecular Biology Grade)

CE174 1 g
EUR 232.8

Hyaluronidase Grade I (Molecular Biology Grade)

CE175 5 g
EUR 920.4

Magnesium acetate - Tetrahydrate (Molecular Biology Grade)

CE190 500 g
EUR 98.4

NADH - Disodium salt (Molecular Biology Grade)

CE198 1 g
EUR 91.2

NADH - Disodium salt (Molecular Biology Grade)

CE199 5 g
EUR 244.8

NADP - sodium salt (Molecular Biology Grade)

CE200 250 mg
EUR 92.4

NADP - sodium salt (Molecular Biology Grade)

CE201 1 g
EUR 190.8

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE202 25 mg
EUR 70.8

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE203 100 mg
EUR 126

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE204 500 mg
EUR 374.4

SSC Buffer (20X) (Molecular Biology Grade)

CE229 1 l
EUR 86.4

XTT sodium salt (Molecular Biology Grade)

CE250 100 mg
EUR 208.8

XTT sodium salt (Molecular Biology Grade)

CE251 500 mg
EUR 612

Tetrahydrofuran

GK1522-1L 1 l
EUR 84

Tetrahydrofuran

GK1522-2500ML 2500 ml
EUR 122.4

Tetrahydrofuran

S-3460 1ML
EUR 38.76

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE100 50 g
EUR 128.4

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE101 100 g
EUR 193.2

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE102 250 g
EUR 387.6

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE103 500 g
EUR 656.4

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE104 1 kg
EUR 1162.8

Tetrahydrofuran, 99.9%, for analysis, unstabilised

GK0126-1L 1 l
EUR 136.8

Tetrahydrofuran, 99.9%, for analysis, unstabilised

GK0126-2500ML 2500 ml
EUR 246

Tetrahydrofuran-d8

S-3461 1ML
EUR 71.82

Agarose LE, Ultra-Pure Molecular Biology Grade, 100 g

41028-100G 100G
EUR 266.4
Description: Minimum order quantity: 1 unit of 100G

Agarose LE, Ultra-Pure Molecular Biology Grade, 25 g

41028-25G 25G
EUR 130.8
Description: Minimum order quantity: 1 unit of 25G

Agarose LE, Ultra-Pure Molecular Biology Grade, 500 g

41028-500G 500G
EUR 862.8
Description: Minimum order quantity: 1 unit of 500G

EDTA solution pH 8.0 (0.5 M) (Molecular Biology Grade)

CE141 500 ml
EUR 87.6

LB-Agar - Powder according to Lennox (Molecular Biology Grade)

CE178 500 g
EUR 109.2

LB-Agar - Powder according to Lennox (Molecular Biology Grade)

CE179 2.5 kg
EUR 301.2

LB-Agar - Powder according to Miller (Molecular Biology Grade)

CE180 500 g
EUR 104.4

LB-Agar - Powder according to Miller (Molecular Biology Grade)

CE181 2.5 kg
EUR 295.2

LB-Medium - Powder according to Lennox (Molecular Biology Grade)

CE182 500 g
EUR 108

LB-Medium - Powder according to Lennox (Molecular Biology Grade)

CE183 2.5 kg
EUR 301.2

LB-Medium - Powder according to Miller (Molecular Biology Grade)

CE184 2.5 kg
EUR 295.2

VOC Tetrahydrofuran Neat

REVOC176N 10MG
EUR 37.62

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT

AGR-LE-100 1/pk
EUR 201.6
Description: Bioscience Mol Bio; Agarose

Bulk Beads Zr 0.1mm Triple-Pure Molecular Biology Grade 250g

HOM3048 250G
EUR 536.94

Bulk Beads Zr 0.5mm Triple-Pure Molecular Biology Grade 250g

HOM3050 250G
EUR 536.94

Bulk Beads Zr 1.0mm Triple-Pure Molecular Biology Grade 250g

HOM3052 250G
EUR 536.94

Bulk Beads Zr 1.5mm Triple-Pure Molecular Biology Grade 250g

HOM3054 250G
EUR 536.94

Bulk Beads Zr 3.0mm Triple-Pure Molecular Biology Grade 300g

HOM3056 300G
EUR 536.94

Tetrahydrofuran, anhydrous, 99.9%, unstabilised

GK8887-100ML 100 ml
EUR 93.6

Tetrahydrofuran, anhydrous, 99.9%, unstabilised

GK8887-1L 1 l
EUR 154.8

Tetrahydrofuran, anhydrous, 99.9%, unstabilised

GK8887-2500ML 2500 ml
EUR 265.2

Tetrahydrofuran, anhydrous, 99.9%, unstabilised

GK8887-500ML 500 ml
EUR 117.6

ELISA kit for General 5-Me-THF (5-Methyltetrahydrofolate)

ELK8059 1 plate of 96 wells
EUR 446.4
Description: A competitive Inhibition ELISA kit for detection of 5-Methyltetrahydrofolate from General in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Methenyl-THF synthetase Recombinant Protein

92-280 0.05 mg
EUR 852.9
Description: 5-formyltetrahydrofolate cyclo-ligase (MTHFS) belongs to the 5-formyltetrahydrofolate cyclo-ligase family. It is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, contributes to tetrahydrofolate metabolism. MTHFS helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion.

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE

AGR-LE-5001 1/pk
EUR 3669.6
Description: Bioscience Mol Bio; Agarose

AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE

AGR-MS-1001 1/pk
EUR 2445.6
Description: Bioscience Mol Bio; Agarose

5-Me-THF ELISA KIT|Human

EF007403 96 Tests
EUR 826.8

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE

AGR-LE-1001 1/pk
EUR 926.4
Description: Bioscience Mol Bio; Agarose

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-1001 1/pk
EUR 4484.4
Description: Bioscience Mol Bio; Agarose

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 50G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-501 1/pk
EUR 2490
Description: Bioscience Mol Bio; Agarose

(3R,4S)-Tetrahydrofuran-3,4-diamine dihydrochloride

abx180099-1g 1 g
EUR 2431.2

(R)-2-(Tetrahydrofuran-3-Yl)Acetic-Acid

abx188023-10g 10 g
EUR 2932.8

VOC Std Tetrahydrofuran 2000ug/mL in MeOH

REVOC176 1ML
EUR 52.44

General 5-Methyltetrahydrofolate (5-Me-THF) ELISA Kit

CEG818Ge-10x96wellstestplate 10x96-wells test plate
EUR 6588.86
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General 5-Methyltetrahydrofolate (5-Me-THF) in serum, plasma and other biological fluids.

General 5-Methyltetrahydrofolate (5-Me-THF) ELISA Kit

CEG818Ge-1x48wellstestplate 1x48-wells test plate
EUR 647.78
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General 5-Methyltetrahydrofolate (5-Me-THF) in serum, plasma and other biological fluids.

General 5-Methyltetrahydrofolate (5-Me-THF) ELISA Kit

CEG818Ge-1x96wellstestplate 1x96-wells test plate
EUR 873.98
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General 5-Methyltetrahydrofolate (5-Me-THF) in serum, plasma and other biological fluids.

General 5-Methyltetrahydrofolate (5-Me-THF) ELISA Kit

CEG818Ge-5x96wellstestplate 5x96-wells test plate
EUR 3572.93
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General 5-Methyltetrahydrofolate (5-Me-THF) in serum, plasma and other biological fluids.

General 5-Methyltetrahydrofolate (5-Me-THF) ELISA Kit

4-CEG818Ge
  • EUR 6649.20
  • EUR 3513.60
  • EUR 874.80
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of General 5-Methyltetrahydrofolate (5-Me-THF) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

General 5-Methyltetrahydrofolate ELISA Kit (5-Me-THF)

RK00608 96 Tests
EUR 625.2

30UL CLEAR PIPET TIPS FOR MOLECULAR DEVICE LIBERTY.

LT-384-R 3840/pk
EUR 2631.6
Description: Robotic Tips; Robotic tips - Axygen

30ul Clear Pipet Tips for Molecular Device Liberty.

LT384R PK19200
EUR 4174.68

Molecular Weight Marker

abx098957-15ul 15 ul
EUR 126

Agarose, Molecular Grade

BIO-41025 500g Ask for price

Agarose, Molecular Grade

BIO-41026 100g Ask for price

Molecular sieves 200g

TIT1072 200G
EUR 564.3

Tetra Butylammonium Fluoride 1M in THF CA 5% Water

TBAF125 2.5L
EUR 1755.6

DiscoveryProbe? Cancer Biology-related Compounds Panel

L1003-5 5 mg/well
EUR 4736.4
Description: A wide range of well-characterized bioactive molecules that covers various targets related to cancer biology, including p53, EGFR and PKC etc. Facilitate your research towards the insights of gene regulation, tumorgenesis and immunotherapy etc.

Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column

FAPDE-holder-for-ion-exchange 1 prep
EUR 189.6

ELISA kit for Human LMWK (Low Molecular Weight Kininogen)

ELK1799 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Low Molecular Weight Kininogen from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human HMWK (High Molecular Weight Kininogen)

ELK2201 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of High Molecular Weight Kininogen from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Mouse HMWK (High Molecular Weight Kininogen)

ELK7788 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of High Molecular Weight Kininogen from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

30uL Black Tips For Molecular Device Liberty N/ST

PIP1678 PK19200
EUR 4289.82

Chitosan, low molecular weight

GC9009-100G 100 g
EUR 141.6

Chitosan, low molecular weight

GC9009-50G 50 g
EUR 91.2

Fitness landscape can be used to describe the evolution of the potential trajectory of cancer, positive selection and mapping of neutral evolution of proto-oncogenes, tumor suppressor, and other functional elements. We also highlight the current challenges to implement these concepts and propose the research area, by incorporating these concepts, identify the mode of new therapies and in cancer susceptibility.