Molecular biology and structure of a novel penaeid shrimp densovirus elucidate convergent parvoviral host capsid evolution

Black tiger shrimp (Penaeus monodon) is a comprehensive decapod crustaceans raised for human consumption. Currently, the virus from two different lineages of parvovirus (PV, family Parvoviridae; subfamily Hamaparvovirinae) infects penaeid shrimp.

Here, the PV was isolated and cloned from Vietnam specimens of P. monodon, Penaeus monodon appointed metallodensovirus (PmMDV). This was the first member of a different lineage third proved to infect penaeid decapods. PmMDV has the unique transcription strategy among PV invertebrates, extensive use of alternative splicing and transcription incorporating elements characteristic of PV-infect vertebrates. The PmMDV protein has no significant sequence similarity with other PV, except for the helicase domain SF3 its nonstructural protein.

The capsid structure, determined by cryoelectron microscope for 3-Å resolution, has a surface morphology similar to Penaeus stylirostris densovirus, despite the lack of significant capsid viral proteins (VP) of sequence similarity. Unlike other PV, VP crease PmMDV without combining βA multimer strand and shown a unique interaction, including the incorporation of cations Ca2 +, attach the N termini below fivefold icosahedral symmetry axis and forming a basket-like pentamer helix bundle. While the order PmMDV VP does not have a canonical domain of phospholipase A2, the structure of a capsid EDTA-treated, determined to 2.8-Å resolution, suggesting a mechanism cation-dependent membrane-penetrating alternative in the N-terminal region.

PmMDV is observed examples of convergent evolution between invertebrates PV capsid structure in connection with the host-driven and unique as PV shows the structure of the cation-sensitive cart / egress endosomal depending on the alternative. Bioinformatics is a very resourceful tool for understanding the evolution of membrane proteins, such as transient receptor potential channel. bioinformatics expert users rely on special scripting and programming skills. Some web servers and standalone tools are available to users nonadvanced is willing to develop projects to understand their system of choice.

 Molecular biology and structure of a novel penaeid shrimp densovirus elucidate convergent parvoviral host capsid evolution
Molecular biology and structure of a novel penaeid shrimp densovirus elucidate convergent parvoviral host capsid evolution

molecular biology and evolution of cancer: from discovery to action.

Cancer development is an evolutionary process. During this process, the growing population of cancer cells undergo limit the ecological niches in the body, such as primary tumor, circulatory system, and metastatic sites vary. heterogeneous population of cancer cells undergo selection for adaptive phenotypes, which form the molecular genetic variations in the same genetic drift. cell lineage underwent convergent evolution towards a phenotype known as hallmarks of cancer that promote cancer initiation, growth, and metastasis.

Efforts to prevent or delay cancer evolution-and-development requires a deep understanding of the underlying process of molecular evolution. Here we discuss the suite concept and tools of the theory of evolution and ecology that can inform-and possibly change-cancer biology in new ways and means. These concepts and tools including comparative research on cancer throughout the different species and the application of phylogenetic approaches to analyze the evolution of tumor progression and metastasis.

Tetrahydrofuran, GlenDry™, anhydrous over molecular sieve

GS8667-2500 2500
EUR 246.9

Tetrahydrofuran, GlenDry™, anhydrous over molecular sieve

GS8667-500 500
EUR 104.1

Tetrahydrofuran (THF)

TC8900 1L
EUR 103.85

Magnesium Acetate Tetrahydrate for molecular biology, 99%

50488 100 Gms
EUR 2.74
Description: Part A

Tetrahydrofuran (THF) for HPLC & UV Spectroscopy, 99.9%

94842 500 ml
EUR 6.71
Description: Part A

Tetrahydrofuran (THF) GC-HS, 99.9%

32661 250 ml
EUR 4.11
Description: Part A

Tetrahydrofuran (THF) (Stabilized) pure, 99.5%

82863 500 ml
EUR 4.52
Description: Part A

Magnesium acetate - Tetrahydrate (Molecular Biology Grade)

CE190 500 g
EUR 98.4

Tetrahydrofuran (THF) Dried, 99.5%, 0.005% water

59167 500 ml
EUR 6.5
Description: Part A

Tetrahydrofuran (THF) (Stabilized) extrapure AR, 99.5%

74497 500 ml
EUR 4.79
Description: Part A

Tetrahydrofuran-d8 (THF-d8) for NMR spectroscopy, 99.5 Atom %D

22698 5 ml
EUR 133.42
Description: Part A

Tetrahydrofuran Mix (THF) - 3 pack of 1.5mL ampules - 1.5ML

ECS-B-047 1.5ML
EUR 121.5

Tetrahydrofuran (THF) (Stabilized) extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.5%

83642 500 ml
EUR 5.2
Description: Part A

EDTA Tetrasodium Salt Dihydrate for molecular biology, 99.5%

45247 100 Gms
EUR 6.16
Description: Part A

Casein, for Molecular Biology

MB279-100G 1 unit
EUR 110.77
Description: Casein, for Molecular Biology

Casein, for Molecular Biology

MB279-500G 1 unit
EUR 387.65
Description: Casein, for Molecular Biology

Tetrahydrofuran

33112-75 500ML
EUR 11.69

Tetrahydrofuran

33113-36 100ML
EUR 9.1

Tetrahydrofuran

33113-65 500ML
EUR 13.09

Tetrahydrofuran

33116-35 500ML
EUR 29.05

Tetrahydrofuran

33125-31 1L
EUR 44.1

Tetrahydrofuran

33139-45 500ML
EUR 63

Tetrahydrofuran

GK1522 500ml
EUR 65.14

Tetrahydrofuran

GK1522-1 1
EUR 39.4

Tetrahydrofuran

GK1522-1L 1 l
EUR 84

Tetrahydrofuran

GK1522-2500 2500
EUR 71.1

Tetrahydrofuran

GK1522-2500ML 2500 ml
EUR 122.4

Tetrahydrofuran

GK1522-500 500
EUR 23.8

Tetrahydrofuran

T293518 1l
EUR 57
Description: 109-99-9

Sucrose for molecular biology

27580 500 Gms
EUR 4.11
Description: Part A

Tetrahydrofuran, 99.9%, for analysis, unstabilised

GK0126 1l
EUR 159.23

Tetrahydrofuran, 99.9%, for analysis, unstabilised

GK0126-1 1
EUR 83.1

Tetrahydrofuran, 99.9%, for analysis, unstabilised

GK0126-1L 1 l
EUR 136.8

Tetrahydrofuran, 99.9%, for analysis, unstabilised

GK0126-2500 2500
EUR 174

Tetrahydrofuran, 99.9%, for analysis, unstabilised

GK0126-2500ML 2500 ml
EUR 246

Tetrahydrofuran 99%

T04810 1L
EUR 238.6

Urea for molecular biology, 99.5%

21113 500 Gms
EUR 6.5
Description: Part A

Tetrahydrofuran - 1ML

S-3460 1ML
EUR 51.3

Xylene for molecular biology, 99.5%

45122 250 ml
EUR 2.81
Description: Part A

Tetrahydrofuran-d8

T293512 1g
EUR 230
Description: 1693-74-9

Tetrahydrofuran-2,2,5,5-d4

T293519 10mg
EUR 64
Description: 20665-63-8

Acetone for molecular biology, 99.8%

27498 500 ml
EUR 3.76
Description: Part A

Glycine for molecular biology, 99.5%

64072 100 Gms
EUR 1.64
Description: Part A

Carbinol for molecular biology, 99.5%

34883 500 ml
EUR 6.23
Description: Part A

Methanol for molecular biology, 99.5%

96446 500 ml
EUR 6.23
Description: Part A

Formamide for molecular biology, 99.5%

30349 500 ml
EUR 5.82
Description: Part A

Imidazole for molecular biology, 99.5%

61510 25 Gms
EUR 5.47
Description: Part A

Chloroform for molecular biology, 99.8%

96764 100 ml
EUR 3.08
Description: Part A

Urea, suitable for molecular biology

GE1210 500g
EUR 53.1

Urea, suitable for molecular biology

GE1210-1 1
EUR 58

Urea, suitable for molecular biology

GE1210-1KG 1 kg
EUR 106.8

Urea, suitable for molecular biology

GE1210-500 500
EUR 33.1

Urea, suitable for molecular biology

GE1210-500G 500 g
EUR 76.8

Tetrahydrofuran, GlenUltra™, analytical grade, for LC

GS2560 1l
EUR 281.92

Tetrahydrofuran, GlenUltra™, analytical grade, for LC

GS2560-1 1
EUR 176.2

Tetrahydrofuran, GlenUltra™, analytical grade, for LC

GS2560-2500 2500
EUR 294

Triethylamine for molecular biology, 99.5%

55221 500 ml
EUR 4.11
Description: Part A

Tetrahydrofuran-d8 - 1ML

S-3461 1ML
EUR 94.5

Ficoll 400® for molecular biology

45460 5 Gms
EUR 15.6
Description: Part B

Nitro Blue Tetrazolium Chloride (Nitro BT) (NBT) for molecular biology, 99%

48898 100 Mg
EUR 6.6
Description: Part B

Agarose UltraPhor for molecular biology

27813 10 Gms
EUR 46.5
Description: Part B

Tetrahydrofuran 〈H2O<50ppm〉

04113-81 1L
EUR 64.4

Tetrahydrofuran 〈H2O<50ppm〉

04113-94 100ML
EUR 14.7

Boric Acid for molecular biology, 99.5%

22311 250 Gms
EUR 4.04
Description: Part A

2-Methyl Tetrahydrofuran

24707-100 100g
EUR 55
Description: 96-47-9

2-Mercaptoethanol ?For Molecular Biology

MB041-100ML 1 unit
EUR 9.02
Description: 2-Mercaptoethanol ?For Molecular Biology

2-Mercaptoethanol ?For Molecular Biology

MB041-500ML 1 unit
EUR 26.47
Description: 2-Mercaptoethanol ?For Molecular Biology

ACES Buffer for molecular biology, 99.5%

27907 5 Gms
EUR 5.4
Description: Part B

Triton X-100 for molecular biology

64518 100 ml
EUR 6.02
Description: Part A

MOPS Buffer for molecular biology, 99%

66043 25 Gms
EUR 10.28
Description: Part B

Vitamin B12 for molecular biology, 97%

77472 250 Mg
EUR 5.78
Description: Part B

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE202 25 mg
EUR 70.8

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE203 100 mg
EUR 126

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE204 500 mg
EUR 374.4

HEPES Buffer for molecular biology, 99.5%

16826 25 Gms
EUR 6.3
Description: Part B

Sodium Azide for molecular biology, 99%

17782 100 Gms
EUR 5.47
Description: Part A

CHAPS Buffer for molecular biology, 99.5%

21420 1 Gms
EUR 10.73
Description: Part B

Agarose Low EEO for molecular biology

36601 10 Gms
EUR 5.1
Description: Part B

PIPES Buffer for molecular biology, 99%

49159 100 Gms
EUR 26.03
Description: Part B

10X MOPS Buffer for molecular biology

68078 100 ml
EUR 4.5
Description: Part B

Agarose High EEO for molecular biology

60645 25 Gms
EUR 13.8
Description: Part B

Jasmonic Acid for molecular biology, 95%

79238 25 Mg
EUR 120.9
Description: Part B

Protamine Sulfate for molecular biology, 90-110%

78349 1 Gms
EUR 22.5
Description: Part B

Oil Red O for molecular biology, 75%

23576 25 Gms
EUR 5.33
Description: Part B

Agarose Medium EEO for molecular biology

10423 10 Gms
EUR 6.75
Description: Part B

Sodium Chloride for molecular biology, 99.9%

33205 500 Gms
EUR 4.52
Description: Part A

Isopropanol (IPA) for molecular biology, 99.8%

38445 100 ml
EUR 1.71
Description: Part A

VOC Tetrahydrofuran Neat - 10MG

REVOC176N 10MG
EUR 52.73

Agarose Low Melting for molecular biology

32417 5 Gms
EUR 22.5
Description: Part B

Ammonium Acetate for molecular biology, 98%

37829 100 Gms
EUR 2.12
Description: Part A

Acetonitrile (ACN) for molecular biology, 99.9%

62006 250 ml
EUR 4.93
Description: Part A

Ethidium Bromide for molecular biology, 95%

93079 1 Gms
EUR 4.58
Description: Part A

3-(Chloromethyl)tetrahydrofuran

B423875 500mg
EUR 800
Description: 89181-22-6

Ammonium Chloride for molecular biology, 99.5%

16992 500 Gms
EUR 4.52
Description: Part A

Ammonium Sulphate for molecular biology, 99.5%

82126 250 Gms
EUR 4.93
Description: Part A

Potassium Acetate for molecular biology, 99.5%

96248 100 Gms
EUR 2.74
Description: Part A

Phenol Crystalline for molecular biology, 99.5%

17286 100 Gms
EUR 3.15
Description: Part A

Sodium Bicarbonate for molecular biology, 99.7%

36328 500 Gms
EUR 5.47
Description: Part A

Glycerol (Glycerine) for molecular biology, 99.5%

62417 100 ml
EUR 2.05
Description: Part A

Potassium Chloride for molecular biology, 99.5%

84984 500 Gms
EUR 6.16
Description: Part A

Dichloromethane (DCM) for molecular biology, 99.9%

41512 250 ml
EUR 3.22
Description: Part A

Acriflavine Neutral for molecular biology, 98%

42428 5 Gms
EUR 3.15
Description: Part B

2-Mercaptoethanol for molecular biology, 99%

83759 100 ml
EUR 5.27
Description: Part A

Artemether Tetrahydrofuran Acetate

A777415 250mg
EUR 244
Description: 181528-64-3

Dithioerythritol (DTE) for molecular biology, 99%

53384 1 Gms
EUR 9.53
Description: Part B

Tetrahydrofuran-2,5-dicarboxylic Acid

T293525 1g
EUR 167

Sodium chloride, suitable for molecular biology

GE0307 1kg
EUR 41.33

Sodium chloride, suitable for molecular biology

GE0307-1 1
EUR 45.2

L-Ascorbic Acid for molecular biology, 99.7%

14116 25 Gms
EUR 1.64
Description: Part A

HEPES Sodium Salt for molecular biology, 99%

32225 25 Gms
EUR 11.85
Description: Part B

meta-Topolin (mT) for molecular biology, 98%

68913 25 Mg
EUR 17.48
Description: Part B

Sucrose, GlenBiol, suitable for molecular biology

GC3201-1KG 1 kg
EUR 90

2,5-Bishydroxymethyl Tetrahydrofuran

B446375 100mg
EUR 170
Description: 104-80-3

Glycogen ex. Oyster for molecular biology, 85%

49740 1 Gms
EUR 7.5
Description: Part B

2-Methyl Tetrahydrofuran pure, 98%

99983 100 ml
EUR 2.26
Description: Part A

2,5-Bis(aminomethyl)tetrahydrofuran

B404300 100mg
EUR 253
Description: 66918-21-6

VOC Std Tetrahydrofuran 2000ug/mL in MeOH - 1ML

REVOC176 1ML
EUR 74.91

Propidium Iodide (PI) for molecular biology, 95%

11195 10 Mg
EUR 17.63
Description: Part B

Acetic Acid Glacial for molecular biology, 99.9%

59788 100 ml
EUR 3.56
Description: Part A

Acrylamide 3x cryst. for molecular biology, 99.9%

61346 100 Gms
EUR 5.47
Description: Part A

Brilliant Blue G-250 for molecular biology

64222 5 Gms
EUR 4.65
Description: Part B

Brilliant Blue R-250 for molecular biology

93473 5 Gms
EUR 4.35
Description: Part B

D-Sorbitol Powder for molecular biology, 98%

99103 500 Gms
EUR 10.33
Description: Part A

Acriflavine Hydrochloride for molecular biology, 98.5%-105%

35188 5 Gms
EUR 4.05
Description: Part B

2-(Tetrahydrofuran-3-yl)acetaldehyde

T293605 2.5g
EUR 1200
Description: 1072-94-2

Tetrahydrofuran, GlenDry™, anhydrous

GS6029 100ml
EUR 213.81

2-(4-Hydroxybutoxy)tetrahydrofuran

H819500 100mg
EUR 295
Description: 64001-06-5

Tetrahydrofuran, GlenDry™, anhydrous

GS6029-1 1
EUR 121

Tetrahydrofuran, GlenDry™, anhydrous

GS6029-100 100
EUR 66.4

Tetrahydrofuran, GlenDry™, anhydrous

GS6029-2500 2500
EUR 223

Tetrahydrofuran, GlenDry™, anhydrous

GS6029-500 500
EUR 88.7

Tetrahydrofuran Fentanyl Hydrochloride

T293476 10mg
EUR 1455

Tris Buffer Superior for molecular biology, 99.9%

37969 100 Gms
EUR 7.59
Description: Part A

Polysorbate 20 (Tween 20) for molecular biology

65296 100 Gms
EUR 10.81
Description: Part A

Tetrahydrofuran-2,5-dicarboxylic Acid (>80%)

T222765 1g
EUR 81
Description: 6338-43-8

Molecular Biology Grade Water for RT-PCR

ML065-1.5ML 1 unit
EUR 7.82
Description: Molecular Biology Grade Water for RT-PCR

10X TE Buffer pH-8.0 for molecular biology

51782 100 ml
EUR 9.45
Description: Part B

10X TAE Buffer pH-8.3 for molecular biology

71989 100 ml
EUR 4.43
Description: Part B

Tris Buffer AR, ACS for molecular biology, 99.9%

71033 100 Gms
EUR 6.02
Description: Part A

10X TBE Buffer pH-8.3 for molecular biology

83170 200 ml
EUR 4.95
Description: Part B

MES Monohydrate Buffer for molecular biology, 99%

26854 25 Gms
EUR 11.03
Description: Part B

Acrylamide 40% aq. solution for molecular biology

29787 250 ml
EUR 20.53
Description: Part A

2-((Tetrahydrofuran-2-yl)methoxy)phenol

T689455 10mg
EUR 201
Description: 41516-19-2

Glutathione Reduced (GSH) for molecular biology, 99.5%

11514 1 Gms
EUR 5.55
Description: Part B

DL-Dithiothreitol (DTT) for molecular biology, 98%

17315 1 Gms
EUR 5.18
Description: Part B

Sodium Acetate Anhydrous for molecular biology, 99%

22342 250 Gms
EUR 3.56
Description: Part A

Dimethyl Sulphoxide (DMSO) for molecular biology, 99.8%

24075 100 ml
EUR 6.02
Description: Part A

Trifluoroacetic Acid (TFA) for molecular biology, 99.9%

40801 100 ml
EUR 22.92
Description: Part A

Ammonium Persulphate (APS) for molecular biology, 99%

65553 25 Gms
EUR 1.64
Description: Part A

Cobalt(II) chloride-1,5-Tetrahydrofuran

GX7281 10g
EUR 139.17

Cobalt(II) chloride-1,5-Tetrahydrofuran

GX7281-10 10
EUR 88.9

Cobalt(II) chloride-1,5-Tetrahydrofuran

GX7281-25 25
EUR 131.1

Tetrabutylammonium Fluoride(1mol/l in Tetrahydrofuran)

08795-12 25ML
EUR 21.7

(2S,3R,4R,5R)-2-(4-Chloro-3-(4-(((S)-tetrahydrofuran-3-yl)oxy)benzyl)phenyl)-5-((R)-1,2-dihydroxyethyl)tetrahydrofuran-3,4-diol

C419220 100mg
EUR 3000
Description: 1620758-32-8

(R)-2-(Tetrahydrofuran-3-Yl)Acetic-Acid

abx188023-10g 10 g
EUR 2932.8

Glutathione Oxidized (GSSG) for molecular biology, 99.5%

22151 250 Mg
EUR 22.5
Description: Part B

Cesium Chloride ultrapure for molecular biology, 99.9%

32355 25 Gms
EUR 18
Description: Part B

Cesium Sulphate extrapure for molecular biology, 99.9%

57455 10 Gms
EUR 7.5
Description: Part B

Guanidine Thiocyanate (GTC) for molecular biology, 99%

80272 100 Gms
EUR 5.47
Description: Part A

Sodium Acetate Trihydrate for molecular biology, 99.5%

88035 250 Gms
EUR 2.33
Description: Part A

Fitness landscape can be used to describe the evolution of the potential trajectory of cancer, positive selection and mapping of neutral evolution of proto-oncogenes, tumor suppressor, and other functional elements. We also highlight the current challenges to implement these concepts and propose the research area, by incorporating these concepts, identify the mode of new therapies and in cancer susceptibility.